Expression Analysis of Vir E2-N-EGFP Mediated by Agrobacterium in Two Solanaceae Plants

In order to make it clear that whether heterologous protein could be transiently expressed in Solanaceae plants,we cloned the Vir E2 gene from agrobacterium by PCR,fused it to the N terminal of EGFP and successfully constructed the plant expression vector p PZP-Vir E2-NEGFP. Vir E2-N-EGFP was expressed in the leaf cells of Capsicum annuum and Lycopersicon esculentum Mill mediated by agrobacterium,with the Nicotiana benthamiana as control. Green fluorescence was observed in the leaf cells of Nicotiana benthamiana and Capsicum annuum,but not in that of Lycopersicon esculentum Mill. The results indicated that the heterologous protein Vir E2-N-EGFP could be successfully expressed in the leaf cells of Capsicum annuum,which provide a basis for research on the function of the pathogeny interacted protein in Capsicum annuum.