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2005•The Journal of Medical Theory and PracticeRequires access

Apoptosis of Human Colonic Cancer Cell Line Induced by Survivin Antisense Oligonucleotide

Wei Wang

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Abstract

Objective:To explore the apoptosis of human colonic cancer cell line LS174T induced by liposome-survivin antisense oligonucleotide.Methods: Survivin antisense oligonucleotide was synthesized and trasfected into LS174T cells by lipofection.Survivin expression was determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western-blotting.The caspase-3 activities were measured.The apoptosis rate was detected by flow cytometry.Results: The mRNA expressions of survivin were inhibited by survivin ASODN(P0.05);we could not detect the expression of survivin protein by Western-blotting after treatment with survivin ASODN;The caspase-3 activity was significantly higher than that of control group;Apoptosis rate reached much higher than that of untreated group.Conclusion:Survivin antisense oligonucleotide can effectively induce apoptosis of human colonic cancer cell line LS174T.

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What this paper is about

Objective:To explore the apoptosis of human colonic cancer cell line LS174T induced by liposome-survivin antisense oligonucleotide.Methods: Survivin antisense oligonucleotide was synthesized and trasfected into LS174T cells by lipofection.Survivin expression was determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western-blotting.The caspase-3 activities were measured.The apoptosis rate was detected by flow cytometry.Results: The mRNA expressions of survivin were inhibited by survivin ASODN(P0.05);we could not detect the expression of survivin protein by Western-blotting after treatment with survivin ASODN;The caspase-3 activity was significantly higher than that of control group;Apoptosis rate reached much higher than that of untreated group.Conclusion:Survivin antisense oligonucleotide can effectively induce apoptosis of human colonic cancer cell line LS174T.

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Available abstract

Objective:To explore the apoptosis of human colonic cancer cell line LS174T induced by liposome-survivin antisense oligonucleotide.Methods: Survivin antisense oligonucleotide was synthesized and trasfected into LS174T cells by lipofection.Survivin expression was determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western-blotting.The caspase-3 activities were measured.The apoptosis rate was detected by flow cytometry.Results: The mRNA expressions of survivin were inhibited by survivin ASODN(P0.05);we could not detect the expression of survivin protein by Western-blotting after treatment with survivin ASODN;The caspase-3 activity was significantly higher than that of control group;Apoptosis rate reached much higher than that of untreated group.Conclusion:Survivin antisense oligonucleotide can effectively induce apoptosis of human colonic cancer cell line LS174T.

Key concepts: Survivin, Apoptosis, Blot, Molecular biology, Oligonucleotide, Cancer research, Cell culture, Flow cytometry

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