Protective effects of salidroside on lipopolysaccharide-induced injury in RAW264.7 cells

AIM: To explore the protective effects and the underlying mechanism of salidroside(SDS) on lipopolysaccharide(LPS)-induced inflammation in murine macrophage RAW264.7 cells.METHODS: Cells were divided randomly into eight groups: control group(A group),LPS group(B group),SDS 5 group(C group),SDS 5+LPS group(D group),SDS 10 group(E group),SDS 10+LPS group(F group),SDS 20 group(G group) and SDS 20+LPS group(H group).After cells were stimulated with LPS(1 μg/ml) for 6 h in the presence or absence of 0-20 μg/ml SDS for 1 h,activation of nuclear factor kappa B(NF-κB) was detected and analyzed by Western blotting.Supernatant was then used for determination of TNF-α,IL-6,and IL-10 levels by ELISA and lactate dehydrogenase(LDH) activity with corresponding detection kit according to the manufacturer' s instructions.Cells were stimulated with LPS(1 μg/ml) for 24 h in the presence or absence of 0-20 μg/ml SDS for 1 h,and cell viability was measured using the MTT assay.RESULTS: MTT assay showed that 1 μg/ml LPS reduced cell viability,but SDS concentration-dependently reversed LPS-induced reduction of cell viability.Western blotting showed that SDS markedly inhibited the activation of NF-κB in LPS-treated cells.SDS concentration-dependently reduced LPS-induced TNF-α,IL-6,and LDH content and increased IL-10 content.CONCLUSION: Salidroside plays an important role in anti-inflammation of murine macrophage RAW264.7 cells induced by LPS.Protection may be correlated with inhibition of inflammatory factors through NF-κB signal pathway and the balance between pro-and anti-inflammatory systems.