Effect of a short hairpin RNA targeting Survivin on the proliferation and apoptosis in osteosarcoma cell line MG-63

[Objective]To construct an expression plasmid of a short hairpin RNA targeting Survivin,and investigate its effect on the expression of Survivin,the proliferation and the apoptosis of human osteosarcoma cell line MG-63 in vitro.[Method]A plasmid of a short hairpin RNA targeting Survivin was constructed,and it was transfered into MG-63 cell line by dosper liposomal method.Morphological change of tumor cells was observed under invert microscope.The expression of Survivin mRNA and protein were examined by RT-PCR and Weston-blot method respectively.The inhibition of the cell proliferation was estimated by MTT method and the colony forming test.The apoptotic cells were stained by acridine orange.[Result]The number of drift cells was increased after transfection,Survivin shRNA could significantly reduce the expressions of Survivin mRNA and protein.The expression of Survivin mRNA and protein after transfection was respectively inhibited by 85.08% and 81.14%,Survivin shRNA also inhibit the growth of the cell by MTT method and the inhibition rate reached 63.41% at 48 h,the apoptosis rate of the cells in the transfected group was 24.54%.[Conclusion]Survivin shRNA could significantly reduce the expressions of Survivin mRNA and protein and inhibit the proliferation of the MG-63 cell,and increase the apoptosis of the cells.