2010•National Medical Frontiers of ChinaRequires access

Autologous osteogenic effect of embryonic stem cells induced by embryonic stem cells

Wang Don

Abstract

Objective To explore how endothelial cells(Ec) induced by embryonic stem cells(embryonic stem cells,ESCs) affect autologous embryonic stem cells when ESCs Transulate into Osteoblas.Method Extract mouse embryonic stem cells and embryonic fibroblast,then induce embryonic stem cells to Ec.Cells were divided into four groups.Group A:embryonic stem cells;Group B:Induce embryonic stem cells;Group C:Induce endothelial cells;Group D:Induce endothelial cells and embryonic stem cells.From Stem cell morphology,Immunofluorescence,Cell value and ALP,then Observe how endothelial cells affect embryonic stem cells when it induced Osteoblast from the enzyme.Result By immunofluorescence it is confirmed that the cell of C group was induced EC.D Group:from MTT detection Cell proliferation in each group have no significant difference in meaning(p 0.05) from Alkaline Phosphatase the Transformation of group D is better than the other groups(p 0.05).Conclusion Endothelial cells induced by Embryonic stem cells into have a clear role when the Embryonic stem cells Transulate into Osteoblast.

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Key concepts: Embryonic stem cell, Stem cell, Endothelial stem cell, KOSR, Cell biology, Amniotic epithelial cells, Embryoid body, Alkaline phosphatase