Relationship of renal cell carcinoma angiogenesis with heparanase and cyclooxygenase-2

Objective To study relationships of tumor growth,metastasis and angiogenesis with protein expressions of cyclooxygenase-2(Cox-2) and heparanase(Hpa) in renal cell carcinoma(RCC).Methods Immunohistochemical staining with StreptAvidin-Biotin Complex(SABC) was employed to determine expressions of Cox-2,Hpa and CD34 in 60 RCC tissues and 30 tumor-adjacent renal tissues.Cox-2 and Hpa expressions and microvessel density(MVD) in RCC tissues and tumor-adjacent renal tissues were analyzed and compared.Relations of MVD expression with clinicopathological parameters and prognosis were analyzed.Results Among 60 RCC tissues,positive rates of Cox-2 and Hpa expressions were 71.7% and 63.3%,respectively,and both of them were significantly higher than those in tumor-adjacent renal tissues(χ2=24.308,χ2=20.089,P0.05).Increased expression of Cox-2 in RCC was associated with tumor size(χ2=4.34,P0.05).Expression of Hpa was not related to tumor size,while it was significantly related to lymphatic metastasis(χ2=12.72,P0.05).MVD were 125.74±35.68 and 70.87±23.64 in RCC with Cox-2-positive and negative expressions,and 138.56±40.74 and 80.88±21.52 in RCC with Hpa-positive and negative expressions,and there were significant differences among them(P=0.001).MVD was related to histological grade,clinical stage and prognosis of RCC(P0.05).Conclusion Enhanced expression of Cox-2 is associated with tumor size.Cox-2 and Hpa can promote angiogenesis.MVD is related to prognosis.