Soluble Expression of Human β-defensin 3 in E.coli and Identification of its Bioactivity

Two oligonucleotide primers were synthesized according to the codon p reference of E.coli to get the whole human beta defensin 3(hBD 3) gene by PCR .The gene was cloned into pGEX 4T 2.Then the recombinant vector pGEX 4T 2 h BD 3 was transformed into E.coli.Clones carrying pGEX 4T 2 hBD 3 was induce d by IPTG.The fusion and soluble induced protein was obtained by repeated cycles of freezing and thawing,then cut by thrombin to get the recombinant hBD 3(rhBD 3).The rhBD 3 showed antibacterial activity to S.aureus by the test of antiba cterial peptide agarose diffusion assay.