The protein expression of HLA-I on colorectal carcinoma

Objective To measure the protein expression of human leukocyte antigen class Ⅰ on colorectal carcinoma. Methods Among 41 colorectal carcinoma cases, 14 cases were well differentiated adenocarcinoma, 17 cases were moderate differentiated ones and 10 were low differentiated ones. HLA-I antigens were detected by immuno-histochemistry using monoclonal antibody against HLA-I antigens. Results The expression of HLA-I on the positive cell membrane and cytoplasmic expression were observed.The positive rate of HLA-I was 36.59% in colorectal carcinoma. Which was 80% in normal tissues.There was a significant difference between two groups (P 0.05) .The expression of HLA-I in cancer tissues was correlated statistically with the degree of differentiation (P 0.05) .The expression in cases with lymph node metastasis was not correlated statistically with those without lymph node metastasis ( P 0.05). Conclusion The expression of HLA-I is lower in colorectal carcinoma than that in normal tissues and positively correlated with histological grade of cancer,but not with lymph node metastasis.