2002•PubMedRequires access

[cDNA array in the establishment of a gene expression profile associated with differentiation inducing the glioma cells].

Lijun Sun, Qiang Huang, Aidong Wang, Qing Lan, Ziwei Du, Gengxi Hu

Abstract

OBJECTIVE: Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible. METHOD: The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot. RESULTS: Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also. CONCLUSION: A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.

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Key concepts: Glioma, Sodium butyrate, Gene, Complementary DNA, Biology, Gene expression, Molecular biology, Cellular differentiation