Isolation and Identification of Osteoclasts from Mice in vitro

Mice were selected as experimental subjects in this study to observe the morphological and biological features of osteoclasts in vitro.The morphological and function of these osteoclasts were observed and identified by phase-contrast microscope,TRAP staining and scanning electron microscope(SEM)in the mouse coculture assay.The results showed that the cells had classic characteristics of osteoclasts :multinucleated giant cells,staining positively for TRAP in cells,forming bone absorptive lacunae on the bone slices.We got a certain amount of bone resorption activity of osteoclasts with the murine coculture assay.