2005•Optics and Precision EngineeringRequires access
Two-laser scanning confocal system for microarray analysis
Zhenqing Li
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Abstract
A dual-fluorescence microarray scanner based on laser confocal principle was constructed to detect the Cy_3-protein or Cy_5-protein dotted slides. The images were constructed according to the fluorescent intensity signals,and enhanced through adopting the technique of morphologic methods to meet the situation in which the fluorescent intensity was very low and the dynamic range of the data was wide relatively.The measuring ways of the signal-to-noise ratio and the sensitivity of the system were put forward,and the measurement result shows the sensitivity of the system is about 0.1 fluo/μm~2.