2014•Chinese Journal of Integrated Traditional and Western NephrologyRequires access
Effect of Tangshenping Capsule on Expression of Nephrin and CD_2AP in Podocytes of Early Diabetic Nephropathy Rats
Wang Ying-cha
Open publisher page 0 citations
Abstract
Objective: To observe the effects of Tang Shen Ping Capsule on the expression of Nephrin and CD2AP in podocytes of diabetic nephropathy( DN) rats. Methods: Seventy-four male Wistar rats were randomly divided into the normal group( n = 10) and the model group( n = 64). The model of diabetes was induced by intraper-itoneal injection of streptozotocin( STZ 45 mg / kg). Then The diabetic rats were randomly divided into 4 groups: the model group,the irbesartan group,the small dose tangshenping capsule group and the high dose tangshenping capsule group. All the rats were gavaged for 14 weeks. The protein and mRNA levels of nephrin and CD2AP in the kidney tissues were also determined by immunohistochemistry and in 1situ hybridization. Then observed under light microscope. RT-PCR detection of kiney nephrin mRNA. Results: After tangshenping capsule intervention,the levels of Nephrin and CD2AP also markedly increased( P 0. 01). Conclusion: Tangshenping capsule might protect kidney against STZ induced injury via increasing the expression of nephrin and CD2AP in podocytes.