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2015•Zhongguo shengwu huaxue yu fenzi shengwu xuebaoRequires access

Hepatocyte Growth Factor-induced Epithelial-mesenchymal Transition in Human Hepatocellular Carcinoma Cells

Yin Song

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Abstract

The epithelial-mesenchymal transition( EMT) is closely associated with the invasion and metastasis of tumors. Hepatocyte growth factor( HGF) can be a main inducer of tumor EMT,but the underlying mechanism is still not clear. In this study,we investigated the effect of Snail on HGF-induced EMT in Hep G2 and Hep 3B hepatocellular carcinoma cells. The migration of Hep G2 and Hep 3B cells were detected by wound healing and transwell assays. The proteins of p-Met,p-AKT,E-cadherin,Vimentin,Fibronectin,and Snail were determined by Western blotting. The mRNA expression of Ecadherin,vimentin,fibronectin,and Snail were determined by real-time RT-PCR. HGF induced Hep G2 and Hep 3B underwent morphological changes from epithelial to mesenchymal appearance with increased migration behavior. HGF1 also up-regulated the expression of mesenchymal marker proteins and downregulated the epithelial markers,with increased level of Snail expression. The EMT induced by HGF could be blocked by knocking down Snail. It suggested that Snail was involved in the invasion andmetastasis of hepatocellular carcinoma cells.

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What this paper is about

The epithelial-mesenchymal transition( EMT) is closely associated with the invasion and metastasis of tumors. Hepatocyte growth factor( HGF) can be a main inducer of tumor EMT,but the underlying mechanism is still not clear. In this study,we investigated the effect of Snail on HGF-induced EMT in Hep G2 and Hep 3B hepatocellular carcinoma cells. The migration of Hep G2 and Hep 3B cells were detected by wound healing and transwell assays. The proteins of p-Met,p-AKT,E-cadherin,Vimentin,Fibronectin,and Snail were determined by Western blotting. The mRNA expression of Ecadherin,vimentin,fibronectin,and Snail were determined by real-time RT-PCR. HGF induced Hep G2 and Hep 3B underwent morphological changes from epithelial to mesenchymal appearance with increased migration behavior. HGF1 also up-regulated the expression of mesenchymal marker proteins and downregulated the epithelial markers,with increased level of Snail expression. The EMT induced by HGF could be blocked by knocking down Snail. It suggested that Snail was involved in the invasion andmetastasis of hepatocellular carcinoma cells.

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Available abstract

The epithelial-mesenchymal transition( EMT) is closely associated with the invasion and metastasis of tumors. Hepatocyte growth factor( HGF) can be a main inducer of tumor EMT,but the underlying mechanism is still not clear. In this study,we investigated the effect of Snail on HGF-induced EMT in Hep G2 and Hep 3B hepatocellular carcinoma cells. The migration of Hep G2 and Hep 3B cells were detected by wound healing and transwell assays. The proteins of p-Met,p-AKT,E-cadherin,Vimentin,Fibronectin,and Snail were determined by Western blotting. The mRNA expression of Ecadherin,vimentin,fibronectin,and Snail were determined by real-time RT-PCR. HGF induced Hep G2 and Hep 3B underwent morphological changes from epithelial to mesenchymal appearance with increased migration behavior. HGF1 also up-regulated the expression of mesenchymal marker proteins and downregulated the epithelial markers,with increased level of Snail expression. The EMT induced by HGF could be blocked by knocking down Snail. It suggested that Snail was involved in the invasion andmetastasis of hepatocellular carcinoma cells.

Key concepts: Hepatocyte growth factor, Vimentin, Epithelial–mesenchymal transition, Snail, Fibronectin, Mesenchymal stem cell, Cancer research, Biology

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