2007•Journal of Southwest China Normal UniversityRequires access

Analysis of Biodegradation Characteristics and Metabolic Pathway of Toluene-degrading Bacteria

Danqun Huo

Abstract

A bacterial strain isolated from waste sludge was identified as Pesudomonas putida according to morphological,physiological experiment.It can use toluene as sole carbon and energy sources.Under experimental conditions,the strain degraded 80%~100%of the added toluene at temperature 20~40℃ and pH for 7.The toluene concentrations under 316mg/L can not significantly affect its removal efficiency.The biodegradation pathway of toluene has formed cis-toluene dihydrodiol by toluene dioxygenase.

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Key concepts: Toluene, Biodegradation, Pseudomonas putida, Strain (injury), Chemistry, Bacteria, Metabolic pathway, Bacterial strain