Studies on the Development of Male- and Female- Gametophytes and Callose Deposit Dynamics in Anmearrhean asphodeloides

The callose appears in the early meiotic prophase in microspore mother cell walls, and deposite no directive. At early tetrad stage callose wall reach the thickest, and begin to disappear at late tetrad stage. Microspore begin to synthesis and depositon callose wall at tetrad phase. At spore-release phase the callose fluorescence is strong. It decline at mid-vacuolate phase, and disappear at pre-mitosis. Callose wall is occurs in the generative cell wall. The formation of callose in the walls during megasporogenesis is similar that in microsporogenesis. It appears in the early meiotic prophase, but there is a markly polarity, always first appear in wall of the chalazal part. At triads stage, callose occurs in the transverse wall, and no in sidewalls. Callose fluorescence decrease starting from the chalazal end of functional megaspore, and disappear at late uninuclear embryo sac phase. It appear again at 2-nuclear embryo sac phase, disappear at mature embryo sac. This study revealed that the callose synthesis is similar in male female-gametophyte in the course of sexual cell development. The embryo sac development of Anemarrhena asphodeloides is the monosporic Polygonum type.