Preparation and Performance of Inorganic Silica Gel-Organic Polymer Mixture Based C_4 Capillary Monolithic Column

Hydrolyzing 3-(trimethoxysilyl) propyl methacrylate(γ-MAPS) and co-condensation polymerizing in the presence of an acid solution,of which the production reacts with butyl methacrylate by double bond polymerization in the presence of a heat initiator,the paper has prepared a novel type of inorganic silica gel-organic polymer mixture based capillary monolithic column.The method has combined the preparation of silica-based monolithic column and organic polymer-based monolithic column.The paper has characterized the structure and pore size distribution of the monolithic material by scanning electronmicroscopy and nitrogen adsorption method,investigated the influences of running voltage,organic additive,concentration and pH of buffer on electroosmotic flow(EOF),separated four neutral hydrocarbons,such as benzene,toluene,naphthalene,and biphenyl using the mixture based monolithic column.The results show that the monolithic column has a typical RP chromatographic retention mechanism.