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Study on formation and regeneration of protoplasts in Aspergillus niger Ni-5k

Ping Zhu, Liang Haiqiu, Hong Zhang, Zhou He-zhi

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Abstract

The effect factors such as enzyme system,mycelium age,temperature and time of enzyme treatment on the formation and regeneration of protoplasts in strain Aspergillus niger Ni-5k were studied.The results showed that the suitable conditions were enzyme concentration 1.0%(containing 0.7% Cellulase,0.2% Snailase and 0.1% Lysozyme),mycelium age 18 h,zymolytic temperature 30°C,time 3 h.The protoplast yield was 10~6 cell/mL,and the regeneration percentage was 39% cultured with double layer medium in 32°C.

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What this paper is about

The effect factors such as enzyme system,mycelium age,temperature and time of enzyme treatment on the formation and regeneration of protoplasts in strain Aspergillus niger Ni-5k were studied.The results showed that the suitable conditions were enzyme concentration 1.0%(containing 0.7% Cellulase,0.2% Snailase and 0.1% Lysozyme),mycelium age 18 h,zymolytic temperature 30°C,time 3 h.The protoplast yield was 10~6 cell/mL,and the regeneration percentage was 39% cultured with double layer medium in 32°C.

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Available abstract

The effect factors such as enzyme system,mycelium age,temperature and time of enzyme treatment on the formation and regeneration of protoplasts in strain Aspergillus niger Ni-5k were studied.The results showed that the suitable conditions were enzyme concentration 1.0%(containing 0.7% Cellulase,0.2% Snailase and 0.1% Lysozyme),mycelium age 18 h,zymolytic temperature 30°C,time 3 h.The protoplast yield was 10~6 cell/mL,and the regeneration percentage was 39% cultured with double layer medium in 32°C.

Key concepts: Protoplast, Aspergillus niger, Mycelium, Cellulase, Lysozyme, Enzyme, Regeneration (biology), Strain (injury)

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