The Study of the Combined Gene Therapy for Nasopharyngeal Carcinoma in vitro

Objective: To investigate the effect of hTERT,C-myc Survivin and VEGF on the human nasopharyngeal carcinoma cell growth,and to investigate the mechanism of short hairpin recombinant plasmid targeting four genes having effect on human nasopharyngeal carcinoma cell growth.Methods: RNAi technology was used to construct a short hairpin recombinant plasmid containing four short hairpin RNAS(shRNA) targeting four genes simultaneously while another plasmid just containing single shRNA against hTERTmRNA,and they were transfected into CNE-2Z cells separately by Liposome;Test groups: Blank control group termed BC Group(no interference),Negative control group termed NC Group(the plasmid without targeted gene),A group(single-gene plasmid against hTERT),B Group(combined plasmid hitting multiple gene);The transfection was detected under the LSCM;Cell viability was detected by using the MTT;The mRNA and protein expression were determined by RT-PCR and western blot-ting.Results: Compared with that in BC group and NC group,the cell proliferation viability decreased both in A group and B,and which was more lower in B group.The mRNA and protein expression level also reduced significantly,and which was more lower in B group.Conclusions: Four genes are involved in the occurrence and development of nasopharyngeal carcinoma.The combined gene therapy has a better effect on the inhibition of Nasopharyngeal carcinoma cell proliferation than the single-gene interference does.