An intervention study of Xuebijing injection(血必净注射液) on peripheral T-lymphocyte subpopulations in patients with severe trauma

Objective To investigate the effect and clinical significance of Xuebijing injection(血必净注射液)on peripheral T-lymphocyte subpopulations in patients with severe trauma.Methods Thirty-three patients with severe trauma were divided into a control group (n=16) and a treatment group(n=17).The patients of two groups were all treated conventionally,and the only difference was that Xuebijing injection was given to the treatment group at the early stage.The CD4+ and CD8+ subpopulations of T-lymphocyte in the peripheral blood were detected respectively at admission of hospitalization,and the 3rd and 5th day after trauma by double antibody labeling and flow cytometry.Results The CD4+ T-lymphocytes and CD4+/CD8+ ratio of peripheral blood in patients with severe trauma all decreased markedly at the 3rd and 5th day after trauma(CD4+:(32.40±6.02)% and(34.19±5.63)%,respectively vs.(45.09±6.57)% in control group,(34.30±5.46)% and(38.55±6.47)%,respectively vs.(46.10±6.02)% in treatment group;CD4+/CD8+ ratio: 1.11±0.20 and 1.15±0.20,respectively vs.1.60±0.20 in control group,1.20±0.22 and 1.32±0.24,respectively vs.1.66±0.24 in treatment group,all P0.01).Furthermore,compared with the control group, the peripheral CD4+ T-lymphocytes and CD4+/CD8+ ratio of treatment group elevated obviously at the 5th day after trauma,the differences being statistically significant((38.55±6.47)% and 1.32±0.24 vs.(34.19±5.63)% and 1.15±0.20 both P0.05).Conclusion In the treatment of patients with severe trauma,on the basis of conventional therapy,the early use of Xuebijing injection can quickly correct the disorder or inhibition of helper/suppressor T-lymphocytes immuno-inflammatory regulating network,and help to maintain a more balanced profile of immunologic function.