Comparing study on the inducing cord blood and peripheral blood derived endothelial progenitor cells into endothelial cells in vitro

Objective:To investigate and compare the feasibility and capacity of human umbilical cord blood,peripheral blood derived endothelial progenitor cells(EPCs) differentiating into endothelial cells in vitro.Method:Mononuclear cells were isolated from human umbilical cord blood and peripheral blood by density gradient centrifugation,CD34~-positive mononuclear cells were selected by magnetic bead cell sorting(MACS).Peripheral blood mononuclear cells(PBMC),umbilical cord blood mononuclear cells(CBMC),CD34~+ cord blood mononuclear cells(MNCCD34~+) were cultured in M-199 medium and induced to expand and differentiate.Morphology of the cells were observed and cell clusters numbers were calculated.Cell surface marker CD31 was assessed by fluorescence activated cell sorter(FACS) analysis.Result:Cell clusters numbers derived from 3×10~7 cells of cord blood were 103.00±10.10,of peripheral blood were 74.25±5.44,of MNCCD34+ were 24.25±6.5. The positive rate for CD31 on cells from CBMC,PBMC,MNCCD34~+,MNCCD34~-,cultured after 10 days,were(76.24±16.54)%,(82.2±9.0)%,(70.03±10.27)%,(36.5±11.78)%,respectively.Conclusion:Greater numbers of cell clusters developed from similar numbers of CBMC than from PBMC,MNCCD34~+ (P0.05).There are no significant difference for the cord blood and peripheral blood derived EPCs to differentiating into endothelial cells in vitro(P0.05),but the majority of cells are from CD34~+ cells.