Effect of Heat Shock Protein 70(HSP70) Expressions in Neuronal Cells with Different Hypoxia time

Objective:To investigate the effect of heat shock protein 70(HSP70) expressions in neuronal cells with different hypoxia time.Methods:Cultured neuronal cells with different hypoxia time were divided into six groups(hypoxia 0h, 0.5h, 1h, 2h, 3h and 4h) after they have been infected with recombinant adenovirus(vAd-HSP70) for 48h.Cells in different groups were treated by hypoxia-reoxygenation, then the expression of HSP70 gene was examined by RT-PCR and Western blotting.Results:The expression of human HSP70 gene was tested in the vAd-HSP70-infected group.After hypoxia-reoxygenation treatment, expression of HSP70 mRNA and protein were at higher level within hypoxia 4h, reached its peak at hypoxia 1h, and then step down regularly.At hypoxia 4h group, the expression of HSP70 was lower than that of the other groups, with statistical significance(P0.05).Conclusions:The expression of HSP70 was at high level when the cultured neuronal cells were treated reoxygenation within hypoxia 2h and may also promote the functional recovery.