The Optimal Dosage of Bone Marrow Mesenchymal Stem Cells Transplantation for Treatment of Hepatic Ischemia-Reperfusion Injury in Rats

Objective To investigate the optimal dosage of bone marrow mesenchymal stem cells(BMSCs)transplantations for treatment of hepatic ischemia-reperfusion injury in rats,and to provide prophase experimental basis for it. Methods BMSCs of Wistar rats were isolated and cultivated by bone marrow adherent culture method. BMSCs of the fourth generation were prepared for cell transplantation. Thrity hepatic ischemia-reperfusion injury models of male Wistar rats were successfully established,and then were randomly divided into blank control group,5×105group,1×106group,2×106group,and 3×106group,each group enrolled 6 rats. The 200 μL cell suspension of BMSCs were transfused into the portal vein with number of 5×105,1×106,2×106,and 3×106separately in rats of later 4 groups,and rats of blank control group were injected with phosphate buffered saline of equal volume. At 24 hours after cell transplantation,blood samples were collected to test aspartate aminotransferase(AST)and alanine aminotransferase(ALT),liver tissues were obtained to test malonaldehyde(MDA),superoxide dismutase(SOD),and nuclear factor-κB(NF-κB)p65 protein.Liver tissues were also used to perform HE staining to observe the pathological changes. Results Compared with blank control group,5×105group,and 3×106group,the levels of AST,ALT,and MDA were lower(P0.05)while activity levels of SOD were higher(P 0.05)in 1×106group and 2×106group,and expression levels of NF-κB p65 protein were lower with the pathological injury of liver tissue improved,but there were no significant differences on levels of AST,ALT,MDA,and SOD(P 0.05),and both of the 2 groups had the similar pathological change. Conclusion The optimal dosage of the BMSCs transplantations after hepatic ischemia-reperfusion injury is 1×106.