Study of the related influencing factors of MCF-7 cells proliferation test in vitro

Objective To study the related influencing factors of MCF 7 cells proliferation test in vitro to provide some criteria for the standard detection of environmental estrogens (EE) by cell proliferation test of MCF 7 cells in vitro . Methods The sensitivity to E 2 stimulation of different sources of MCF 7 cell lines was detected. Cell proliferation test was performed on the screened sensitive cells in different culture conditions. Results In the estrogen free medium, C cell line of MCF 7 was more sensitive to E 2 stimulation than A and B cell lines ( P 0.01). The phenolsulfonphthalein in medium and the endogenous estrogen in NBS had stimulating effect on the cell proliferation of MCF 7. The longer MCF 7 was cultured in estrogen free medium, the more sensitive was the cell to the stimulation of estrogen( P 0.01). Conclusion Evaluation of the estrogenic activity by MCF 7 cell proliferation test in vitro may be influenced by many factors.