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Effects of LyGDI on radiosensetivity in A549 cells

Xin-Wen Zhou

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Abstract

OBJECTIVE:To investigate the effect of X-ray on radiosensetivity in A549 cell line of stable transfected with LyGDI and the relative mechanism.METHODS: Growth curve was used to measure changes of cell proliferation.Flow cytometry was used to determine cell cycle distribution and apoptotic cell rate.Clony formation assay was used to measure radiosensetivity of the A549 with different phenotypes.RESULTS: The cell line of stable transfected with LyGDI got a lower proliferation(F=212.55,P0.01) and survival rate(F=7.56,P0.05) after irradiation with different doses of X-ray compared with control cell line.Radiation-induced G2 arrest was abrogated in A549-LyGDI cell line(F=6.29,P0.05) and the apoptosis induced by X-irradiation in A549-LyGDI line was enhanced(F=18.45,P0.01).CONCLUSIONS: It showed that LyGDI induced cell apoptosis upgraded sensitivity to radiation in A549 cell line.Abrogation of radiation-induced G2 arrest can be part of the mechanism.

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OBJECTIVE:To investigate the effect of X-ray on radiosensetivity in A549 cell line of stable transfected with LyGDI and the relative mechanism.METHODS: Growth curve was used to measure changes of cell proliferation.Flow cytometry was used to determine cell cycle distribution and apoptotic cell rate.Clony formation assay was used to measure radiosensetivity of the A549 with different phenotypes.RESULTS: The cell line of stable transfected with LyGDI got a lower proliferation(F=212.55,P0.01) and survival rate(F=7.56,P0.05) after irradiation with different doses of X-ray compared with control cell line.Radiation-induced G2 arrest was abrogated in A549-LyGDI cell line(F=6.29,P0.05) and the apoptosis induced by X-irradiation in A549-LyGDI line was enhanced(F=18.45,P0.01).CONCLUSIONS: It showed that LyGDI induced cell apoptosis upgraded sensitivity to radiation in A549 cell line.Abrogation of radiation-induced G2 arrest can be part of the mechanism.

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Available abstract

OBJECTIVE:To investigate the effect of X-ray on radiosensetivity in A549 cell line of stable transfected with LyGDI and the relative mechanism.METHODS: Growth curve was used to measure changes of cell proliferation.Flow cytometry was used to determine cell cycle distribution and apoptotic cell rate.Clony formation assay was used to measure radiosensetivity of the A549 with different phenotypes.RESULTS: The cell line of stable transfected with LyGDI got a lower proliferation(F=212.55,P0.01) and survival rate(F=7.56,P0.05) after irradiation with different doses of X-ray compared with control cell line.Radiation-induced G2 arrest was abrogated in A549-LyGDI cell line(F=6.29,P0.05) and the apoptosis induced by X-irradiation in A549-LyGDI line was enhanced(F=18.45,P0.01).CONCLUSIONS: It showed that LyGDI induced cell apoptosis upgraded sensitivity to radiation in A549 cell line.Abrogation of radiation-induced G2 arrest can be part of the mechanism.

Key concepts: A549 cell, Apoptosis, Flow cytometry, Cell cycle, Cell culture, Cell growth, Transfection, Radiosensitivity

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