2003•PubMedRequires access

[RT-PCR method for detecting the expression of transforming growth factor-beta1 gene in lung tissues of silica-treated mice].

Wenjie Ji, Lei Yang, Zhenglun Wang, Jia-shun Ding, Cang Liu, He Hanzhen

Abstract

OBJECTIVE: To investigate the gene expression of transforming growth factor-beta(1) (TGF-beta(1)) in lung tissues of silica-treated mice. METHODS: The experimental mice were divided into control and silica group. 0.2 g/kg body weight of silica was injected intratracheally in silica group. Samples of lung tissue were collected 1, 3, 5, 7, 14 and 28 d after injection. RT-PCR method was used to analyze the gene expression of TGF-beta(1) in lung tissue of silica-treated mice. RESULTS: The expression of TGF-beta(1) gene in lung tissue elevated from the 3rd day (1.20 +/- 0.15) and the peak value was on the 7th day (1.74 +/- 0.19). Then the expression decreased from the 14th to 28th day. But there was still higher than control until the 28th day. CONCLUSION: TGF-beta(1) may play an important role in silica-induced pulmonary fibrosis.

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Key concepts: Lung, Gene expression, Transforming growth factor, Pulmonary fibrosis, Gene, Chemistry, Real-time polymerase chain reaction, BETA (programming language)