Gene expression of isoflavonoid synthesis related enzymes in soybean seedlings

In order to identify the effect of cadmium stress on the accumulation of isoflavonoid in soybean,the content of isoflavonoid in leaves and roots were measured and the expression of genes of isoflavonoid synthesis related enzymes in roots treated with 100 μmol / L CdCl2 for different time by quantitative RT-PCR.The results showed that the content of isoflavonoid content in soybean leaves increased with Cd concentration.When treated with 100 μmol / L CdCl2 for 5 d,the isoflavonoid content in roots increase markedly.The expressions of genes involved in isoflavonoid synthesis,including IFS2(isoflavone synthase),PAL(phenylalanine ammonia lyase),C4H(cinnamic acid 4-hydroxylase),4CL(4-coumarate: coenzyme A ligase),CHS(chalcone synthase),CHI(chalcone isomerase) and CHR(chalcone reductase),increased obviously when treated for 24 h,and the trends.Changes were similar to the accumulation of isoflavonoid content in roots treated with 100 μmol / L CdCl2,indicating that soybean could cope with cadmium stress through improving isoflavonoid accumulation caused by boosting gene expression of isoflavonid related enzymes.