Protective Effect of Propofol on Intestine after Hemorrhagic Shock and Resuscitation

Objective: To determine the protective effect of propofol on intestine after hemorrhagic shock and resuscitation in rats when used for a short period of time. Methods: Thirty Wistar rats were randomized into 3 groups,sham operation group (C group), resuscitation group (HS-R group) and propofol group (P group). There were 8 rats in each group. The animal model of hemorrhagic shock and resuscitation was established. Following resuscitation, the rats of HS-R group were infused normal saline at 1 mL·kg-1·h-1 with infusion pump for 2 hours, and rats of P group were infused propofol at 1 mL·kg-1·h-1 for 2 hours. The level of blood diamine oxidase (DAO) was measured in rats of groups. The intestinal tissue of 5 cm was taken. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using 3 cm of the tissue by xanthinoxidase method and thiobarbituric acid colorimetric technique. The other 2 cm of intestinal tissue was used to do HE staining to observe the changes of intestinal pathological histology. Results: The levels of MDA and DAO were higher in HS-R group and P group than those in C group (P 0.05),and were lower in P group than those of HS-R group(P 0.05). The level of SOD was lower in HS-R and P groups than that of C group (P 0.05), and it was higher in P group than that of HS-R group (P 0.05). The pathological grades of intestine were higher in P group and HS-R group than those in C group (P 0.05), but were lower in P group than those of HS-R group (P 0.05). Conclusion: The intestinal tissue was significantly damaged after hemorrhagic shock and resuscitation.Propofol can reduce this damage by against the oxygen free radicals in short-term.