In vitro effect of cyclosporine A on juvenile Schistosoma mansoni labeled by AF18 (5-N-octadecanoyl aminofluorescein).

OBJECTIVE: To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity. METHODS: Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomula and labeling of transformed schistosomula with AF18, then observe schistosomula under fluorescence microscope. RESULTS: Schistosomula of different groups labeled by AF18 were damaged by cyclosporine A in vitro. CONCLUSION: Cyclosporine A increases the uptake of AF18 by schistosomula in vitro which is dose-dependent, and decreases the parasite surface membrane fluidity.