hVEGF165/pcDNA3.1 Expression in Neonate Mice Myocardial Cells

Objective To investigate the transfection and expression of recombinant plasmid human vascular endothelial growth factor 165/pcDNA3.1(hVEGF165/pcDNA3.1) in myocardial cells,and to build foundation for gene therapy and cell therapy of coronary artery disease(CAD).Methods Myocardial cells were cultured in vitro and transfected by hVEGF165/pcDNA3.1 with liposome;then transient expressed protein was detected by reverse transcriptase-polymerase chain reaction(RT-PCR),immunochemistry and Western blotting.Results A strap as hVEGF165 was obtained by RT-PCR,the protein of hVEGF165 was found in myocardial cells by immunochemistry and in supernatant by Western blotting.Conclusion The recombinant plasmid hVEGF165/pcDNA3.1 can be expressed in myocardial cells,and may be used in studying CAD by gene therapy and cell transplantation.