Preparation of high specific antibody against human C1q/TNF-related protein-1

Objective To prepare specific antibody against human C1q/TNF-related protein-1( hCTRP1). Methods A Cterminal antigenic peptide of hCTRP1 was synthesized and injected into the Newzealand rabbits. The antibody was purified by affinity chromatography column. Results High titer antisera against the hCTRP1 was prepared and the antiserum' s titration was over 1 ∶ 64 000 by indirect ELISA. The specificity of the purified antibody by affinity chromatography was identified by Western blot and ELISA. Conclusion The high titer antiserum is prepared by injection for rabbits and the specificity of the antibody purified from the antiserum is determined.