The anti-tumor effects of TNF-related apoptosis-inducing ligand on myeloma in nude mice

Objective To investigate the anti-tumor effects of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) on mouse with inoculated myeloma.Methods Thirty-six male BALB/C mice were inoculated with myeloma cells(9.25×109·L-1,0.2 mL) under the skin of left hips.These 36 mice were randomly divided into 6 groups: control group(Group A),high dose intrperitoneal injection group(Group B1),low dose intraperitoneal injection group(Group B2),high dose peri-tumor injection group(Group C1),low dose peri-tumor injection group(Group C2),and preventive high dose intraperitoneal injection group(Group D).Human recombinant TRAIL were given peritoneally or peri-tumor with different doses(high dose,1.5 mg/kg;low dose,0.5 mg/kg) in different group,except for Group A.Tumor size and weight were assessed after 2 weeks,and further observed with HE staining.The cell cycle and apoptosis index of SP2/0 cells were analyzed by flow cytometry.Results Significantly higher efficacy was revealed in Group C than that in Group B,while so was in high dose treatment than low dose.However,no significant superiority was observed in Group D than Group B1.Furthermore,TRAIL induced tumor cell apoptosis in a dose-dependent manner.Conclusion TRAIL can induce apoptosis of SP2/0 cells,inhibit the growth of myeloma.The efficacy is correlated with route of administration and dosage.