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A New Method for Apoptosis Detection by Multi-parameter Flow Cytometry

Shan Wang, Liang Xueying, Li Ning, Chunxi Zhou, Li-Fang Yu, Jie Liao

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Abstract

To develop a method of analyzing apoptosis using multi-parameter flow cytometry, HL60 cell line was incubated with Annexin V-FITC/PI after exposure to etoposide, and the apoptosis of HL60 cell was monitored by flow cytometry. The result showed that the percentage of apoptosis cell increases with the time of exposure to etoposide. In conclusion, Annexin V FITC/PI method can not only detect special proteins, but also monitor the integrity of cell membrane. Multi-parameter flow cytometry is a rapid, easy and accurate method for the detection of apoptosis.

About this research paper

What this paper is about

To develop a method of analyzing apoptosis using multi-parameter flow cytometry, HL60 cell line was incubated with Annexin V-FITC/PI after exposure to etoposide, and the apoptosis of HL60 cell was monitored by flow cytometry. The result showed that the percentage of apoptosis cell increases with the time of exposure to etoposide. In conclusion, Annexin V FITC/PI method can not only detect special proteins, but also monitor the integrity of cell membrane. Multi-parameter flow cytometry is a rapid, easy and accurate method for the detection of apoptosis.

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Available abstract

To develop a method of analyzing apoptosis using multi-parameter flow cytometry, HL60 cell line was incubated with Annexin V-FITC/PI after exposure to etoposide, and the apoptosis of HL60 cell was monitored by flow cytometry. The result showed that the percentage of apoptosis cell increases with the time of exposure to etoposide. In conclusion, Annexin V FITC/PI method can not only detect special proteins, but also monitor the integrity of cell membrane. Multi-parameter flow cytometry is a rapid, easy and accurate method for the detection of apoptosis.

Key concepts: Flow cytometry, Apoptosis, Annexin, Cytometry, Etoposide, Pi, Medicine, Annexin A5

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