Optimization of anthocyanin extraction from roses by using response surface methodology

To optimize the anthocyanin extraction from roses, On the basis of single-factor test, the mathematical regression model was established about the dependent variable (extraction rate of anthocyanin from roses) and independent variables(the power of ultrasonic, the concentration of acidic ethanol solution, the temperature of ultrasonic,the time of ultrasonic)through Box-Benhnken center composite design and response surface methodology. The optimum extraction conditions as follows: the power of ultrasonic 500 W, the concentration of acidic ethanol solution 55%, the temperature of ultrasonic 40 ℃, the time of ultrasonic 10 min. Under these conditions, the extraction rate of anthocyanin from roses was 6.24 mg/g.