The influence of various deep hypothermic circulatory arrest treatments on brain ATPase

Objective:To observe the effects of deep hypothermic circulatory arrest treatments on brain ATPase and its construction.Methods:Eighteen test dogs were randomly divided into three groups: the group of deep hypothermic circulatory arrest(DHCA),the group of DHCA with retrograde cerebral perfusion(RCP),and the group of DHCA with intermittent antegrade cerebral perfusion(IACP).The temperature of the dogs were cooled down to 18℃ through cardiopulmonary bypass,then the circulatory arrest was practiced on for 90 min.Before the circulatory arrest,after the circulatory arrest and after re-circulation,blood samples were obtained for ATPase and lactate tests.When finishing the surgery,the hippocampus was removed from the dog brain,and the transmission electron microscope was used to observe the changes in the ultrastructure of brain and nerve cells.Results:After circulatory arrest,DHCA and RCP groups showed an obvious decrease in ATPase,and an obvious increase in lactate than front circulatory arrest.After circulatory arrest,there was no obvious difference in ATPase compared to pre-circulatory arrest;the lactate amount was obvious increase in 45 minute after circulatory arrest.Conclusion:The cerebral ischemic injuries would occur when the period of DHCA is long enough.RCP treatment could provide protection for the brain,but it is more likely to cause dropsy in brain and nerve cells.IACP treatment shows better influence in brain protection.