Fermentation of enriched-selenium of black fungus

The test was fermentation of enriched-Selenium of black fungus.It was carried by adding sodium selenite(Na2SeO3)as Selenium source.This provided basis for the development and utilization of organic selenium polysaccharide,etc.We used three different carbon and nitrogen cultures at 26℃,200 rpm for fermented liquid.Accord to polysaccharide content and biomass at different time,we determined optimal medium(per litre)of glucose 20 g,bean cake powder 5g,magnesium sulfate 0.5g,potassium dihydrogen phosphate 2g.The enrichedSelenium fermentation of black fungus was carried out by adding different concentrations of sodium selenite in the optimal medium.It compared with no adding sodium selenite of black fungus.The results showed that the Selenium content,biomass of mycelium and polysaccharide content in the fermented liquid increased along with selenium concentration between 10-50mg/L.The highest value was 50mg/L with 10.48μg/g,9.37g/L and 20.3g/L respectively.When selenium concentration was more than 50mg/L they decreased on the contrary.Results expressed black fungus had a good ability to enriched-Selenium.But the selenium concentration in the fermented liquid affected enriched-Selenium effect,growth of mycelium and polysaccharide content.If appropriate amount of sodium selenium was added,it was helpful to absorb sodium selenium for mycelium,promote mycelium growth and improve polysaccharide content.If the amount of sodium selenium was more than the appropriate amount,they can be inhibited.