2008•Shiyong yixue zazhiRequires access

Ursolic acid-induced apoptosis of glioma cell line U251 and its mechanism

Wang Pen

Abstract

Objective To investigate the role of ursolic acid(UA) in the apoptosis of glioma cell U251 in vitro and its mechanism.Methods Cell culture was performed.Then the effects of UA on the cell cycle were observed by flow cytometry,the change of DNA was observed by agarose gel electrophoresis,the expressions of COX-2,Bax,and Bcl-2 were detected by Western blot,and prostaglandin E2(PGE2) level was measured by radioimmunoassay.Results UA significantly induced the apoptosis of the cell.PGE2 level and both COX-2 and Bcl-2 expressions were declined,while Bax expression did not differ significantly.Conclusion UA can markedly induce the apoptosis of the cell U251,whose mechanism is associated with arresting cell cycle,inhibiting COX-2 expression to reduce PGE2 production,and downregulating Bcl-2 expression.

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Key concepts: Apoptosis, Ursolic acid, Cell cycle, Flow cytometry, Western blot, Cell, Agarose gel electrophoresis, Chemistry