Cloning and Construction of Human Hepatitis B Virus PreS1/S2/S Gene

Objective To construct recombinant plasmid encoding preS1/S2/S antigen of HBV and to investigate the effect of preS1/S2/S antigen on the mitogen activated protein kinase (MAPK) p38 signaling pathway. Methods The specific primers were designed and synthesized to amplify the DNA fragments encoding preS1/S2/S antigen gene from plasmid pHBV adr by PCR. After being digested with restriction endonuclease, Hind III and BamHI, and purification, the purified preS1/S2/S gene fragments were inserted into pcDNA3.1, and transformed into E.coli DH5α. Then the recombinant plasmid were identified by digesting with restriction endonuclease and DNA sequencing. Results The fragments digested with endonuclease were as large as the predicted results while its DNA sequencing was the same with the reported sequence. Conclusion The recombinant plasmid pcDNA3.1 S1/S2/S was successfully constructed.