Killing effects of HSV-TK gene on human glioma mediated by cationic liposome

Objective To observe HSV TK/ACV system killing human glioma transferred with cationic liposome.Methods The eukaryotic expressing vectors pCR3 TK cloned containing the HSV TK gene were transferred into TJ905 cells with cationic liposome,Lipofectamine.After transfection,G418 was used to select the positive clones.The killing of TJ905 glioma cells transfected with HSV TK was examined by MTT under various concentration of ACV.Results The increasing sensitivity level of the TK gene transferred glioma cell to ACV treatment was confirmed.ACV had a specific suppression and killing effects on the glioma cells transfected with TK gene.Conclusion Cationic liposome,Lipofectamine is safe,simple and effective in transferring gene.This indicates that HSV TK/ACV gene therapy of glioma may be studied by cationic liposome transferring PCR3 TK.Such treatment may be used as an innovative method for brain tumor therapy.