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2011•Tianran chanwu yanjiu yu kaifaRequires access

Effect of Julibroside J_8 on Apoptosis of Human Microvascular Endothlial Cell

Hua Hui

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Abstract

HMEC-1 cells were treated with different doses of Julibroside J8 and different time.The inhibition of cell growth was detected by using SRB assay.The TUNEL and DAPI fluorescent staining were measured in cells.Annexin-Ⅴ/PI double staining was used to measure the cell apoptosis rate by flow cytometry.The results showed that Julibroside J8 could inhibit the growth of HMEC-1 and induce the cell apoptosis.The biggest inhibition rate was up to 75.6% and the apoptosis rate was about 32.32%,respectively.

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What this paper is about

HMEC-1 cells were treated with different doses of Julibroside J8 and different time.The inhibition of cell growth was detected by using SRB assay.The TUNEL and DAPI fluorescent staining were measured in cells.Annexin-Ⅴ/PI double staining was used to measure the cell apoptosis rate by flow cytometry.The results showed that Julibroside J8 could inhibit the growth of HMEC-1 and induce the cell apoptosis.The biggest inhibition rate was up to 75.6% and the apoptosis rate was about 32.32%,respectively.

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Available abstract

HMEC-1 cells were treated with different doses of Julibroside J8 and different time.The inhibition of cell growth was detected by using SRB assay.The TUNEL and DAPI fluorescent staining were measured in cells.Annexin-Ⅴ/PI double staining was used to measure the cell apoptosis rate by flow cytometry.The results showed that Julibroside J8 could inhibit the growth of HMEC-1 and induce the cell apoptosis.The biggest inhibition rate was up to 75.6% and the apoptosis rate was about 32.32%,respectively.

Key concepts: DAPI, Apoptosis, Annexin, TUNEL assay, Flow cytometry, Staining, Pi, Cell

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