ScholarLens Back to search
1999•Zhonghua neifenmi daixie zazhiRequires access

Effect of thyroid hormone on Go protein α subunit in hypothalamus of neonatal rat expression

Yuan Chen

Open publisher page 0 citations

Abstract

Objective To study the distribution of Go protein α subunit (Goα) mRNA in hypothalamus of neonatal rat and the effect of thyroid hormone on its expression. Methods Non radioactive in situ hybridization technique was used to observe Goα mRNA distribution in the hypothalamic nuclei of 14 and 21 day Wistar rats, both normal and affected by thyroid hormone deficiency. Results (1) Goα mRNA was mainly located in arcuate nucleus (AR) and ventromedial hypothalamic nucleus (VMH) of 14 or 21 day rats; (2) The expression of Goα mRNA in the hypothalamic nuclei of 21 day rat was significantly weaker than that of 14 day rat; (3) Perinatal hypothyroidism could significantly increase Goα mRNA levels in AR and VMH of 14 and 21 day rats. Conclusion Goα mRNA is characteristically distributed and expressed in AR and VMH of neonatal rat, and the transcription of Goα gene in these nuclei can be down regulated by thyroid hormone during development of rat brain.

About this research paper

What this paper is about

Objective To study the distribution of Go protein α subunit (Goα) mRNA in hypothalamus of neonatal rat and the effect of thyroid hormone on its expression. Methods Non radioactive in situ hybridization technique was used to observe Goα mRNA distribution in the hypothalamic nuclei of 14 and 21 day Wistar rats, both normal and affected by thyroid hormone deficiency. Results (1) Goα mRNA was mainly located in arcuate nucleus (AR) and ventromedial hypothalamic nucleus (VMH) of 14 or 21 day rats; (2) The expression of Goα mRNA in the hypothalamic nuclei of 21 day rat was significantly weaker than that of 14 day rat; (3) Perinatal hypothyroidism could significantly increase Goα mRNA levels in AR and VMH of 14 and 21 day rats. Conclusion Goα mRNA is characteristically distributed and expressed in AR and VMH of neonatal rat, and the transcription of Goα gene in these nuclei can be down regulated by thyroid hormone during development of rat brain.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the distribution of Go protein α subunit (Goα) mRNA in hypothalamus of neonatal rat and the effect of thyroid hormone on its expression. Methods Non radioactive in situ hybridization technique was used to observe Goα mRNA distribution in the hypothalamic nuclei of 14 and 21 day Wistar rats, both normal and affected by thyroid hormone deficiency. Results (1) Goα mRNA was mainly located in arcuate nucleus (AR) and ventromedial hypothalamic nucleus (VMH) of 14 or 21 day rats; (2) The expression of Goα mRNA in the hypothalamic nuclei of 21 day rat was significantly weaker than that of 14 day rat; (3) Perinatal hypothyroidism could significantly increase Goα mRNA levels in AR and VMH of 14 and 21 day rats. Conclusion Goα mRNA is characteristically distributed and expressed in AR and VMH of neonatal rat, and the transcription of Goα gene in these nuclei can be down regulated by thyroid hormone during development of rat brain.

Key concepts: Internal medicine, Endocrinology, Hypothalamus, Messenger RNA, In situ hybridization, Thyroid, Hormone, Arcuate nucleus

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of thyroid hormone on Go protein α subunit in hypothalamus of neonatal rat expression — Research Paper | ScholarLens