Nonspecific Phospholipase C2 in Arabidopsis Possessing The Activity of Phosphatidylcholine Specific Phospholipase C

There are six NPCs in Arabidopsis.The results of GUS staining showed that NPC2 expressed mainly in meristematic zone,elongation zone and vascular tissue of maturation zone of root tip;it also expressed in developing leaves,but its expression decreased with the maturing of leaves.The activity of the mutant npc2-1 was 47.7% lower than that of wild type and could be recovered at different levels by complementary plants.So it indicated that NPC2 possessed the activity of PC-PLC.Although NPC2 gene expressing at the loci of lateral root formation correlated closely with the lateral root formation induced by IAA,the mutant had no obvious difference with the wild type treated by exogenous IAA in lateral root density,main root length and total lateral root length.