Relationship between distribution and drug resistance of class I integron on clinical isolates of K pneumoniae

Objective To investigate the distribution,structure and inducing drug resistance of the class I integron on K pneumoniae.Methods PCR method was used to detect integrase gene(intI)and product of variable region of positive strain was performed on sequencing.Sensitiveness of experimental strains to 14 kinds of antibiotics was detected with microdilution method.Results IntI was identified in 38 strains of plasmid DNA on K pneumoniae(43.2%).The most drug resistance gene cassettes were aad A2 and dfr 17.The MIC of integron positive groups were statistically significantly higher than negative groups.Conclusion Class I integrons were existed in K pneumoniae widely.There was preferably relationhip between strains with gene cassettes and drug resistance phenotype.Gene cassettes mediate bacteria drug resistance.The main cause of drug resistance of integron positive strain to aminoglycosides sulfonamides was drug resistance gene cassettes.