Effect of Mcl-1 Antisense Oligonucleotide on WM451 Cell Proliferation and Apoptosis

Objective To explore the effect of liposomal transfection of Mcl-1 antisense oligonucleotide(Mcl-1 AS-ODN) on WM451cell proliferation and apoptosis.Methods Mcl-1 AS-ODN was transfected into a metastatic human melanoma cell line WM451 with lipofectamine 2000.Mcl-1 expression was analyzed by RT-PCR,Western blotting and immunohistochemistry.Cell viability was assessed by MTT assay,apoptosis was evaluated by flow cytometry and electron microscopy.Results The expression level of Mcl-1 mRNA and protein was decreased in AS-ODN group,whereas there was no significant difference in S-ODN group and NS-ODN group,compared with control group.MTT assay demonstrated that Mcl-1 AS-ODN could reduce the viability of WM451cells.Electron microscopy showed that WM451 cells exhibited characteristic morphologic changes of apoptosis after incubation with Mcl-1 AS-ODN.The apoptosis rate of WM451 cells treated with AS-ODN was 13.6%,which is higher than that of control(6.3%) by flow cytometry.Conclusion Mcl-1 AS-ODN can downregulate Mcl-1 expression,inhibit WM451 cells proliferation and induce apoptosis,which is a promising target for antisense therapy of melanoma.