Gametophyte development of Leptorumohra quadripinnata

The spores of the Leptorumohra quadripinnata were cultured in MS medium.The spore germination,gametophyte development and formation of young sporophytes were observed in detail by microscope.The result indicates that the mature spores are black-brown,monolete,little draped surface and horsebean-shaped in equatorial view and elliptical in polar view.The spores germinate about 8 days after sowing,the germination type is Vittaria-type and the gametophyte development is Aspidium-type.The prothallial plates form about 20 days after sowing.The young prothallia develop about 30 days after sowing,they are not symmetry temporarily,but the mature prothallia are papilionaceous.Profuse unicellular hairs occur in the whole surface and the margin of the prothallium.The sex organs form about 50 days after sowing.The antheridia look like pellets and are formed by three cells.The archegonial neck is often formed by 5 rows of cells.Young embryos can be seen from the prothallium in one month after fecundation.