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2009•Journal of Shenyang Medical CollegeRequires access

Differentiation from rat mesenchymal stem cells into chondrocytes in vitro

Li Na

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Abstract

Objects: To induce bone marrow mesenchymal stem celts or bone marrow stroma celts(MSCs)to differentiate directionally into chondrocytes in vitro and then identify the differentiated cells.Methods:MSCs were obtained from bone marrows of healthy rabbit and then the cultured MSCs at the 4 passage were induced by TGF,dexamethasone(Dex),bFGF and Vitamin C(Vit C).Later,the expression of Col Ⅱ in the induced MSCs cell were detected by RT-PCR.Results:The induced cells exhibited a chondrocyte·like morphology.PCR showed that Col Ⅱ was only expressed in the induced cells.Conclusion:MSCs can differentiate into chondrocytes under the induction of TGF,dexamethasone(Dex),bFGF and Vitamin C(Vit C).

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What this paper is about

Objects: To induce bone marrow mesenchymal stem celts or bone marrow stroma celts(MSCs)to differentiate directionally into chondrocytes in vitro and then identify the differentiated cells.Methods:MSCs were obtained from bone marrows of healthy rabbit and then the cultured MSCs at the 4 passage were induced by TGF,dexamethasone(Dex),bFGF and Vitamin C(Vit C).Later,the expression of Col Ⅱ in the induced MSCs cell were detected by RT-PCR.Results:The induced cells exhibited a chondrocyte·like morphology.PCR showed that Col Ⅱ was only expressed in the induced cells.Conclusion:MSCs can differentiate into chondrocytes under the induction of TGF,dexamethasone(Dex),bFGF and Vitamin C(Vit C).

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Available abstract

Objects: To induce bone marrow mesenchymal stem celts or bone marrow stroma celts(MSCs)to differentiate directionally into chondrocytes in vitro and then identify the differentiated cells.Methods:MSCs were obtained from bone marrows of healthy rabbit and then the cultured MSCs at the 4 passage were induced by TGF,dexamethasone(Dex),bFGF and Vitamin C(Vit C).Later,the expression of Col Ⅱ in the induced MSCs cell were detected by RT-PCR.Results:The induced cells exhibited a chondrocyte·like morphology.PCR showed that Col Ⅱ was only expressed in the induced cells.Conclusion:MSCs can differentiate into chondrocytes under the induction of TGF,dexamethasone(Dex),bFGF and Vitamin C(Vit C).

Key concepts: Mesenchymal stem cell, Chondrocyte, In vitro, Bone marrow, Dexamethasone, Stem cell, Stem cell transplantation for articular cartilage repair, Biology

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