Nuclear factor-kappa B expression in rats following intracerebral hemorrhage and effect of astragalus polysaccharide on it

Objective To investigate the expression of nuclear factor-κB(NF-κB) around the hematoma by the intervension of APS in rat, to study mechanisms of APS protecting brain and its effect on inflammation after intracerebral hemorrhage. Methods ICH models was induced in rats by injection ofⅦtype collagenase into the right globas pallidus. APS was used as the intervension drug. Immunohistochemisty was used to investigate the dynamic changes of the expression of NF-κB in the tissue surrounding hematoma in ICH rats. Ultrastructures of the cells around the hematoma were observed with transmission electron microscope. Results Compared with model group, the scores of neurological behavior in treatment group rats were decreased significantly at 3 day and 5 day (P 0.05),NF-κB positive cells were decreased significantly at 6h,1 day,3 day and 5 day(6h,1d P0.05,3d,5d P 0.01). The pathological changes of the treated group were milder than the model group on 3d postoperative. Conclusion APS can inhibit the activation of NF-κB and effectively alleviate inflammatory response. It can accelerate the recover of neurological function deficit after ICH.