An experimental study on transgenes into CHO cell line by polyethylenimine coated albumin microbubble

Objective:To improve efficiency of transgenes by albumin microbubble,which is a non-virus gene delivery vector.Method:Polyethylenimine coated albumin microbubble(PAMB) was prepared by sonicating the mixture of human albumin,polyethylenimine and glucose.The transgene efficiency of polyethylenimine,polyethylenimine +albumin,PAMB and Lipofectamine 2000 was compared by detecting the positive cell ratio of green fluorescent protein(GFP) using flow cytometry.Results:The average efficiency of transgene by polyethylenimine,polyethylenimine+albumin,PAMB and Lipofectamine 2000 was(13.75±4.88)%,(5.20± 1.15)%,(49.17±6.75)% and(53.72±5.69)%,respectively.The efficiency of PAMB group was higher than that in polyethylenimine group(P 0.05),however,there was no statistical significance between PAMB group and Lipofectamine 2000 group(P 0.05).Conclusion:Albumin microbubble modified by polyethylenimine is highly effective on transgene,and it is a useful non-virus gene delivery vector.This study may provide a new method for gene transfection in vivo.