2011•Zhongguo shouyi zazhiRequires access
Analyzing molecular polymorphism of Escherichia coli isolated from goats by randomly amplified polymorphic DNA
Yang Wei
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Abstract
Randomly amplified polymorphic DNA(RAPD) was used to study the molecular polymorphism of pathogenic Escherichia coli(E.coli) strains isolated from goat in Guangxi.Four random primers were screened from 8 random primers for showing preferably polymorphic amplification of 10 strains E.coli.Totally 18 RAPD bands were identified,however,there was no common RAPD band among the 10 E.coli strains.Nx 31 strain and Nx32 strains were known to be the same isolate by serological typing, however,RAPD bands of two strains exhibited distinct differences,indicating that RAPD had higher distinguishability than serological typing.