Effects of Hydroquinone on Methylation of XPD Gene in K562 Cells

Objective: To investigate the effect of different dose of hydroquinone on methylation of xeroderma pigmentosum group D( XPD) in K562 cells. Methods: K562 cells were treated with 0,15,30 and 60 μmol / L of hydroquinone solution for 48 h repeatedly. The inhibition of hydroquinone on K562 cell proliferation was studied by MTT assay. It was first handled by bisulfite then adopting sequencing PCR( BSP) to test methylate level of XPD. Observing the cell survival rate and gene methylation rate. Results: After being treated with 0,15,30 and 60 μmol / L of hydroquinone solution,K562 cells survival rates were( 100. 00 ± 0. 00) %,( 85. 46 ± 0. 60) %,( 63. 46 ± 7. 02) % and( 51. 20 ± 6. 49) % respectively. Comparing 15 μmol/L group and 0 μmol/L group,the differences were not statistically significant( P 0. 05); comparing 30 μmol / L and 60 μmol / L group,the differences were statistically significant( P 0. 05). XPD gene methylate levels were: 1. 03%( 3 /290),0. 34%( 1 /290),0. 34%( 1 /290) and 0. 70%( 2 /290). Comparing 15,30,60 μmol / L group and0 μmol / L group,the differences were not statistically significant( χ2= 1. 531,P 0. 05). Conclusion:Hydroquinone has obvious inhibiting effect on K562 cells growth,but it has no effect on the XPD gene methylate level.