The effect of hyperbaric oxygen (HBO) on cytokines in the brain tissues and blood-brain barrier after cerebral ischemia-reperfusion in mice

Objective To investigate the effect of hyperbaric oxygen (HBO) on cytokines in the brain tissues and blood-brain barrier after cerebral ischemia-reperfusion in mice. Methods Using conscious mice cerebral ischemia-reperfusion models, 0.25MPa (ATA) HBO were applied 5 times during the reperfusion period, and an injection of 2% Evans blue into tail veins was conducted an hour before the animals were sacrificed. The levels of IL-1β,TNF-α,IL-6,IL-8 and content of Evans blue in the brain tissue were examined by using ABC-ELISA method and spectrophotometry. Results The levels of the cytokines mentioned above and content of Evans blue were successively increased ( P 0.05) in the ischemia referfusion group as compared with those in the sham surgery group. The levels of cytokines and content of Evans blue in brain in the HBO group are similar to those in the the sham surgery group ( P 0.05 ). The levels of cytokines and content of Evans Blue in the HBO+ischemia-reperfusion group were successively decreased as compared with those in the ischemia-reperfusim group ( P 0.05, P 0.01). Conclusion HBO ( 0.25 MPa 5 times) has significant effect of reducing the levels of cytokines and the permeability of blood-brain barrier, but has little effect on normal brain tissues.